BIOL250

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BIOL250

Cellular and Molecular Neurobiology Laboratory BIOL250 FA

Crosslistings: NS&B250

Not Currently Offered

In recent years, an explosion of new immunological, biochemical and microscopic techniques have revolutionized the way neuroscientists study brain structure and function. The goal of this project-based laboratory course is to introduce students to some of the modern cellular and molecular approaches currently used to study the organization and development of the brain. Students will work in pairs to carry out a project focusing on characterization of a panel of monoclonal antibodies previously generated against the developing central nervous system. During the course, students will learn to propogate cell lines in culture, section brain tissue and stain it with antibodies using fluorescence and light microscopic immunocytochemistry, and analyze proteins by SDS-polyacrylamide gel electrophoresis and Western blotting.

MAJOR READINGS

Readings to be taken from current scientific
literature.

EXAMINATIONS AND ASSIGNMENTS

Laboratory notebook, lab reports, class presentations, 3-4 short essays, lab method practical exam.

ADDITIONAL REQUIREMENTS and/or COMMENTS

The time in the laboratory and outside readings are estimated to take approximately 6-8 hours/week. Because of the project-oriented nature of the course, students will be expected to return to the lab at various times during the week to complete experiments. Permission of instructor required for enrollment in course. Unless preregistered students attend the first class meeting or communicate directly with the instructor prior to the first class, they will be dropped from the class list. NOTE: Students must still submit a completed Drop/Add form to the Registrar's Office.

COURSE FORMAT: Discussion Laboratory Seminar

REGISTRATION INFORMATION

Level: UG Credit: 1.00 Gen Ed Area & Dept: NSM BIOL

Prerequisites: BIOL205 and BIOL206 or NS&B213

Last Updated on MAR-22-1999

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