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Academic Year 2005/2006


Laboratory in Cellular and Molecular Neurobiology
BIOL 250 FA

Crosslistings:
NS&B 250

In recent years, an explosion of new immunological, biochemical and microscopic techniques have revolutionized the way neuroscientists study brain structure and function. The goal of this laboratory course is to introduce students to cutting edge laboratory techniques currently used to study the nervous system. Students will learn sterile tissue culture; how to section brain tissue and stain with antibodies using immunochemistry; how to analyze proteins by SDS-polyacrylamide gel electrophoreseis and Western blotting; and how to detect neurochemical events associated with cell death. Class time will also be devoted to analysis of experimental design and hypothesis testing.

MAJOR READINGS

THE RAT NERVOUS SYSTEM: AN INTRODUCTION TO PREPARATORY TECHNIQUES by J. P. Cassella (Author), J. Hay (Author), S. J. Lawson (Author), Publisher: John Wiley & Son Ltd; 1st edition (January 15, 1997) ISBN: 0471969672

A BEGINNER'S GUIDE TO SCIENTIFIC METHOD
by Stephen S. Carey, Edition: Paperback, Publisher: Wadsworth Publishing; 2 edition (June 27, 1997) ISBN: 0534528430

FLUORESCENCE MICROSCOPY (MICROSCOPY HANDBOOKS, 40) by H. J. Tanke, Brian Herman, Edition: Paperback, Publish er: Springer Verlag; 2nd edition (May 1998) ISBN: 0387915516

EXAMINATIONS AND ASSIGNMENTS

Laboratory notebook, lab reports, 2 short scientific essays, lab method practical exam, poster presentation of research projects.

ADDITIONAL REQUIREMENTS and/or COMMENTS

The time in the laboratory and outside readings are estimated to take approximately 8-10 hours/week. Because of the project-oriented nature of the course, students will be expected to return to the lab at various times during the week to complete experiments.

COURSE FORMAT: Laboratory

REGISTRATION INFORMATION

Level: UGRD    Credit: 1    Gen Ed Area Dept: NSM BIOL    Grading Mode: Graded   

Prerequisites: ([MB&B205 or BIOL205] AND [BIOL206 or MB&B206]) OR ([MB&B225 or BIOL225] AND [BIOL206 or MB&B206]) OR [NS&B213 or BIOL213] OR [BIOL182 or MB&B182] OR [MB&B195 or BIOL195] Links to Web Resources For This Course.

Last Updated on MAR-30-2006


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