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Academic Year 2003/2004

Laboratory in Cellular and Molecular Neurobiology
BIOL 250 SP
Crosslistings:
NS&B 250

In recent years, an explosion of new immunological, biochemical and microscopic techniques have revolutionized the way neuroscientists study brain structure and function. The goal of this laboratory course is to introduce students to cutting edge laboratory techniques currently used to study the nervous system. Students will learn sterile tissue culture; how to section brain tissue and stain with antibodies using immunocytochemistry; how to analyze proteins by SDS-polyacrylamide gel electrophoresis and Western blotting; how to detect neurochemical events associated with cell death; and how to identify newly generated cells. Several labs are devoted to training students on use of fluorescent, confocal and electron microscopes. Classtime will also be devoted to ethics case studies and readings presenting both the pros and cons for animal use in research.

MAJOR READINGS

Readings to be taken from current scientific literature.

EXAMINATIONS AND ASSIGNMENTS

Laboratory notebook, lab reports, class presentations, lab method practical exam, poster session.

ADDITIONAL REQUIREMENTS and/or COMMENTS

The time in the laboratory and outside readings is estimated to take approximately 5-10 hours/week. Because of the project-oriented nature of the course, students will be expected to return to the lab at various times during the week to complete experiments. Permission from instructor required for enrollment in this course.

COURSE FORMAT: Laboratory

REGISTRATION INFORMATION

Level: UGRD    Credit: 1    Gen Ed Area Dept: NSM BIOL    Grading Mode: Graded   

Prerequisites: (MB&B205 AND BIOL206) OR (MB&B225 AND BIOL206) OR NS&B213 Links to Web Resources For This Course.

Last Updated on MAR-19-2004

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