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Academic Year 2003/2004
Laboratory in Cellular and Molecular Neurobiology
BIOL 250 FA
In recent years, an explosion of new immunological, biochemical and microscopic techniques have revolutionized the way neuroscientists study brain structure and function. The goal of this laboratory course is to
introduce
students to cutting edge laboratory techniques currently used to study the nervous system. Students will learn sterile tissue culture; how to section brain tissue and stain with antibodies using immunocytochemistry; how
to analyze proteins by SDS-polyacrylamide gel electrophoresis and Western blotting; how to detect neurochemical events associated with cell death; and how to identify newly generated cells. Several labs are devoted to
training
students on use of fluorescent, confocal and electron microscopes. Classtime will also be devoted to ethics case studies and readings presenting both the pros and cons for animal use in research.
MAJOR READINGS
THE RAT NERVOUS SYSTEM: AN INTRODUCTION TO PREPARATORY TECHNIQUES by J. P. Cassella (Author), J. Hay (Author), S. J. Lawson (Author), Publisher: John Wiley & Son Ltd; 1st edition (January 15, 1997) ISBN: 0471969672
A BEGINNER'S GUIDE TO
SCIENTIFIC METHOD
by Stephen S. Carey, Edition: Paperback, Publisher: Wadsworth Publishing; 2 edition (June 27, 1997) ISBN: 0534528430
FLUORESCENCE MICROSCOPY (MICROSCOPY HANDBOOKS, 40) by H. J. Tanke, Brian
Herman, Edition: Paperback, Publish
er: Springer Verlag; 2nd edition (May 1998) ISBN: 0387915516
EXAMINATIONS AND ASSIGNMENTS
Laboratory notebook, lab reports, 2 short scientific essays, lab method practical exam, poster presentation of research projects.
ADDITIONAL REQUIREMENTS and/or COMMENTS
The time in the laboratory and outside readings are estimated to take approximately 8-10 hours/week. Because of the project-oriented nature of the course, students will be expected to return to the lab at various times
during the week to complete
experiments.
COURSE FORMAT:
Laboratory
REGISTRATION INFORMATION
Level:
UGRD
Credit:
1
Gen Ed Area Dept:
NSM BIOL
Grading Mode:
Graded
Prerequisites:
(MB&B205 AND BIOL206) OR (MB&B225 AND BIOL206) OR NS&B213
SECTION 01
- Instructor(s): Naegele,Janice R.
- Instructor's Course Page
- Times: ....R.. 01:10PM-05:00PM; Location: SCIE92
- Reserved Seats: (Total Limit: 10)
- SR. major: 3 Jr. major: 5
- SR. non-major: 0 Jr. non-major: 0 SO: 2 FR: 0
Special Attributes:
- Curricular Renewal: Quantitative Reasoning, Speaking
Links to Web Resources For This Course.
Last Updated on MAR-19-2004
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Copyright Wesleyan University, Middletown, Connecticut, 06459